JKSSB VARIOUS POSTS JKSSB: Syllabus for the Post of Clinical Staff for casualty bed

JKSSB: Syllabus for the Post of Clinical Staff for casualty bed

Jammu and Kashmir Services Selection Board has advertised various Posts via Notifications 01, 02, 03, 04 and 05 of 2019. Syllabus for the Post of Junior Staff Nurse

    • Theory



    • Introduction
    • Different parts of the human body, common Anatomical terms, Anatomical Positions and important
    • Animal Cell
    • Tissue of the body, classification and function
    • Primary tissues of the


    ü Skeletal System

    • Joints & Movements
    • Muscle & Monce


    ü Gastro-intestinal System

    • Mouth and Pharynx
    • Salivary Glands and Tonsils
    • Oesophagus and Stomach
    • Location of different organs in the Abdomen in situ
    • Liver and Gall Bladder
    • Spleen and



    ü Genito-Urinary System

    • Kidney
    • Ureters, Bladder and Urethra
    • Male Reproductive System
    • Female Reproductive System


    ü Respiratory System

    • Thoracic, Pleura and Lungs


    ü Cardio Vascular System

    • Heart and Pericardium
    • Arterial System
    • Venous and Lymphatic System


    ü Nervous System

    • Meaning and cerebrospinal fluid
    • Brain, Spinal cord and the


    ü Loco-Motor System

    • Parts of upper Limb :- Bones Land marks and important vessels



    ü  Theory

    ü Blood

    • Composition and General function of Blood
    • Description of Blood cells :- Normal Counts and
    • Anti-congulants


    ü Cardio-Vascular System

    • Function of heart and blood
    • Circulation :- Systemic Circulation Pulmonary


    ü Respiratory System

    • Name of the Structure involved in respiration and their
    • External and Internal respiration. How respiration and expiration are brought
    • Transport of O2 and CO2 in the
    • Definition of respiratory Rate, Tidal Volume, Vital Capacity, Cyanosis,


    ü Excretory System

    • Functions of Kidney
    • Formation & Composition of Urine Normal and abnormal


    ü Skin

    • Functions of skin


    ü Digestive System

    • Composition and functions of saliva, Mastication and
    • Functions of Stomach, Composition of Gastric Juice Pancreatic Juice, Bile and Succus entericus.


    ü Endocrine Glands

    • Definition, name and the hormones secreted by
    • Major action of each

    ü Reproductive System

    • Male Genital System
    • Female Genital System
    • Names of Primary and Accessory Sex organs in male and Female. Secondary Sexual characters in male and
    • Functions of ovary, formation of Ova, actions of ovarian
    • Functions of Testis – Spermatogenesis and actions of


    ü Blood Group

    • ABO and Rh. Basis for classification, basis for determination, importance and Blood


    ü Cerebrospinal Fluid

    • Formation, composition and


    Ø Practical

    • Demonstration of parts of body(Bony) landmarks on the surface
    • Identification of cells and basic
    • Skeletel System, Identification of Bones and Joints
    • Demonstration of Interior of Thorox with organs in
      • Respiratory System and Pleurae
      • Heart and Blood Vessels


    • Demonstration and Identification of various organs with in abdomen
      • Liver and Gall Bladder
      • Peritoneum stomach and
    • Male Genital System
    • Female Genital System
    • Central Nervous System, Spinal Cord and Site of Lumber Puncture examination will be :-
    • Identification of bones or parts of skeletal system
    • Identification of basic tissues under the microscope
    • Identification of certain organs and Viva
    • Surface marking of any of the important
    • Identification of sites of blood vessels or muscles for injections and site of lumbar


    • Microscopic – Usage, maintenance and Minor repairs
      • Behaviour of RBC in isotonic, Hypotonic and Hypertonic Sodium Chloride Solution
    • Identification of Blood Cells Focused under Microscope :-
      • RBC
      • Various types of WBC
      • Platelets
    • To obtain samples of Plasma and Serum
    • Preparations of Anti-Coagulants : double oxalate and Sodium CCitrate
    • Hacmatocrit
    • Identification of ruled area in Neubauer’s Chamber RBC and WBC Pipettes and Wintrobes and Westergren Pipettes
    • Demonstration of Normal Constituents of Urine and Abnormal Constituents E.G Glucose  and Protein
    • Record



    Ø Theory

    • Elementary knowledge of Inorganic Chemistry Atomic Weight Molecular  weight, Equivalent weight –Acids, basis and Salts Indicators Molar Solutions, Buffer Solution, Titration (Acid Base) Definition of Solution. Methods of expressing  concentration  – Dilution.
    • Elementary knowledge of organic Chemistry – organic Compounds. Aliphatic  and Aromatic. Alcohols, Aldehydes, ketones, Amines, Esters, Phenol, Acids Colloids
    • Elementary of Analytical Chemistry I Instrumentation, centrifuge Balances, Colorimeter, Spetrophtometer, Flamephotometer, Flurimeter
    • Aims and Scope Biochemistry.
    • Carbohydrates :- Importance, Definition, Classification some
    • Proteins – Aminoacids, essential amino acids, peptides, denaturation of proteins, Physiologically important proteins, functions of plasma
    • Lipid – Definition, Classification, Steroids,
    • Nucleic Acids- DNA and RNA their
    • Haemoglobin
    • Enzymes and Co-Enzymes
    • Gastric Juice collection
    • Carbohydrate – Metabolism – elementary aspects, definition of Glucolysis, Glycogenolysis Hormonal regulation of Blood Sugar Diabetes-Mellitus – Ketosis, Gcosuria, Renal Glycosuria,
    • Metabolism of Lipids – elementary aspects, Triglyeerides, Cholestrol, Plasma Lipoproteins- Ketone bodies and
    • Protein Metabolism – Formation of Urea, Creatinine Proteinuria Edema, Transaminases
    • Water and Mineral Metabolism – Dehydration, Calcium Phosphorus, Sodium, Potassium, Chloride, Iron, Iodine their physiological functions and disease
    • Harmones – definition, functions of some important
    • Blood and cerebrospinal Fluid functions of Blood &
    • Urine Normal and abnormal


    Ø Practical

    • Basic Techniques :-
    • Cleaning of Glassware
    • Preparation of Chromic acid wash solution
    • Preparation of saturated
    • Types and use of
    • Balance types and
    • Preparation of percent solution / volume / volume components (V/V)
    • Preparation of percent solution weight by volume (W/V)
    • Preparation of Molar
    • Preparation of Buffer
    • Indicators pH, determination of unknown
    • Preparation of Normal
    • Titration – (Acid Base) Preparation of Primary
    • Titration preparation of Normal Solutions
    • Preparation of Protein
    • Use and maintenance of
    • Colorimeter – types, components, use and
    • Colorimetry – Choice of
    • Spectrophotometer components and use
    • List of spare parts of equipments
    • Distillation of water-setting up Glass Distillation Unit and Metal water Distillation
    • Diagnostic tests on Urine :-
      • Collection and
      • Physical characteristics and specific gravity
    • U
      • Qualitative tests    for   urea,   Uric    Acid, Creatinine, Calcium, Phosphorus, Sodium, Potassium and
      • Urea clearance and Creatinine
    • Abnormal Constituents of
      • Qualitative test for Sugar, Albumin, Ketone Bodies, Blood,  Bile Salt and Bile
    • Da…..tests on
      • Collection and preservation of Blood, Serum and
      • Estimation of Blood
      • Glucose Tolerance
    • Non-Protein nitrogenous compound :
      • Determination of Serum Urea, Uric Acid and Creatinine
    • Determination of Serum Protein
      • Albumin, Globulin, Fibrignogen & AG
    • Serum



    • Serum Enzymes :




    • Serum Bilirubin :


    • Serum Lipids :
    • Determination of Na*, K* and
    • Determination of inorganic Phosphorus
    • Determination of


    • Determination of transminases (GOT and GPT)
    • Determination of Phosphatase (Alkaline phosphate and acid Phosphate)
    • Determination of Amylase


    • Determination of total and direct bilirubin


    • Lipid Profile
    • Determination of Serum Cholesterol
    • Liver Function
    • Diagnostic test on other body fluids
    • Gastric juice :-
      • Test of Hcl. Blood and Starch
      • Free and Total acidity
      • Gastric function
    • Cerebrospinal Fluid
    • Determination of sugar
    • Determination of Proteins
    • Determination of Proteins
    • Pandy’s
    • Kidnet or renal function test :
      • Importance of renal function tests

    o  Tests


    • Concentration / Specific Gravity test
    • Dilution test
    • Urea Clearance Test
    • Creatinine Clearance test
    • Laboratory Maintenance and empowerment
      • Quality Control
      • Automation and Kits
      • Laboratory



    ü Theory


    Requirement and use of Common Laboratory Equipment.
    • Incubator, Hot Air Oven, Autoclave, Water bath, Anacrobic jar Vaccum Pump, Media Pouring Chamber, refrigerator, Centrifuge


    • Principal, Operation, Care and Use of Microscope


    Sterilization and Disinfection.
    • Classification and Genaral principles of Sterilization. Physical Chemical and Mechanical Methods Disposal of contaminated media, Syringes, Glossware, Apparatus.


    Classification and Morphology of Bacteria.
    • Brief Outline of :-
      • Structure of cell, capsule, Flagella and spores
      • Growth Bacteria
      • Nutrition of
    ·        Staining of Bacteria :
    • Simple, Grams, Ziehl-Neelsen, Albert, Spore Stain
    • Composition and preparation of Staining reagents
    ·        Cultivation of Micro Organisms – I (In Detail)
    • Classification of Media, Composition of Laboratory culture meida and Special Media
    ·        Cultivation of Micro Organisation – II (In Detail)
    • Identification of Bacteria :
      • Cultural Characters, Bio Chemical reactions and
      • Normal Flora of micro Organisms in the human
      • Gam positive and Gram Negative co….Staphylo…..Penumococcus Neisseria (in brief)



    ·        Gram negative Bacilli :
    • Salmonella, Shigella, E.Coli, Klebsiella, Protein, Pseudomonas Vibro cholera Haemophilus (In brief)
    ·        Gram Positive Bacilli
    • Aerobic
      • Corynebacterium diphtheria
      • Mycobacterium tubercoulosis and Mycobacterium
    •         Anacrobic bacilli – Clostridia
    • Antibiotic Sensitivity test – Principles and methods of determination of
      • Candida, Asperigillus. Dermatophytes
    ·        HIV & AID
    • Brief Account
    • Immunity, Antigens, Antibodies and Antigen antibody reaction and their applications in diagnosis of
    • Principles, Procedures    and    Diagnostic    significance    of   agglutination    Neutralization and complement fixation reactions.
    • Collection and processing of Clinical materials like Sputum. Urine Swabs, Stool, Blood CSF and


    • Parasitology :
    Brief Account of :-  Morphology, Life Cycle, Pathogenicity and  Laboratory Diagnosis  of :-
    1. Hystolytica, E, Coli Giardia. Trichomonas. Plasmodia Leishmania, Hook worn Round worn, Whip worm. Tape worm, Echinococcus granulosus, granulosus, Dracunculus, Wucheraria Bancrofti.


    Ø Practical

    ü Microbiology Practicals :

    • Personal safety and
    • Emergency treatment for Laboratory accidents
    • Care and Cleaning of Glasswares, Syringes, apparatus, preparation of Pasteur pipettes and sealing of
    • Operation of Autoclave, Incubator, Water bath, PH meter, Scitz filter. Ph comparator, Vacuum
    • Operation of Anaerobic
    • Urine C/s & Colony
    • Pus C/S.
    • Sputum C/S and Blood C/S.
    • Sterilization, Packing Loding of materials in Autoclace, Hot Air Oven
    • Handling care of Microscope
    • Preparation of various Media Pouring and Storage
    • Hanging Drop Method
    • Collection of Clinical Materials – Blood Urine Stool Pus Swab, Throat Swab
    • Receipt and Recording of specimen in the Laboratory and dispatch of specimen to referenc laboratory for
    • Gram Stain Z.N Stain Albert’s Stain, Capsule Staining
    • Incolution of Clinical Material in Media
    • Isolation of Organisms in pure
    • Antibiotic Sensitivity test
    • Disposal of contaminated materials
    • Fungus Examination by wetmount of
    • Animal house training collection of blood of sheep and


    ü Parasitology Practicals :

    • Collection, Preservation and Transporation of fear material for examination of
    • Preparation of stained and unstained feeal material for
    • Concentration Techniques of Stool
    • Preservation of Parasites
    • Identification of Ova and Cyst in stool. Occult Blood
    • Parasites Blood
    • Serology :-
    • Widal
    • VDRL
    • Ra Test
    • CRP test
    • ASO test
    • Elisa for IIIV – 1 &
    • HBsAg (Australia Antigen)
    • Pregnancy
    • Diagnostic Skin Test
    • Mauntoux Test
    • Casoni’s Test



    • Theory
      • Introduction of Haematology
      • Collection of Blood
      • Antieoagulants
      • Red Cell Count :
        • Haemocytometer
        • Methods
      • White Cell Count. (Total Leucocyte Count:
        • Morphology of White
        • Normal
        • Romanowsky Stains
        • Staining
        • Counting Methods
      • Absolute Eosi Nophil Count :
      • Erytrocyte Sedimentation Rate (ESR)
        • Westergren’s Method
        • Wintrobe’s Method
        • Factors effecting ESR
        • Importance and Limitations
        • Normal
      • Packed Cell
        • Macro and Micro Methods
        • Normal
      • Haemoglobin Estimation and its clinical Importance
    • Red Cell
    • Calculations and
    • Retienlocyte Count :
      • Methods
      • Appearance
      • Normal
    • Sickle Cell Preparation.
    • Osmotic Fragility Test
      • Scorning
      • Qualitative and Quantitative Test
      • Normal
      • Factors allocating fragility
      • Interpretation
    • Peripheral Blood Film
    • Preparation of Bone Marrow Smears
    • Coagulation







    • Urimanalysis




    • CSF Examination


    • Semen Analysis



    • Coomb’s
    • Process of Coagulation
    • Factors of Coagulation
    • Tests of Coagulation
      • Bleeding time
      • Whole Blood Coagulation Time
      • Clot Retraction Test
      • Toorniquet Test
      • Platelet Count


    • Normal
    • Physical Examination
    • Chemical Examination
    • Microscopic Examination


    • Normal and abnormal Cell Count


    • Physical Preterition
    • Motility
    • Morphology


    ü Histotechnology :

    • Introduction
    • Cell, Tissues and their functions
    • Examination Methods of Tissues and Cells
    • Fixation of Tissue :
    • Classification of fixatives :
      • Simple fixatives and their
      • Micro anatomical
      • Cytolofical
    • Tissue Processing
    • Collection of specimen
    • Labeling and Fixation
    • Dehydration
    • Cleaning
    • Impregnation
    • Section Cutting




    • Staining





    • Decalificatation




    • Exfoliative Cytolgy
    • Microtomes and their Knives
    • Techniques of Section cutting
    • Mounting of Sections
    • Frozen Section


    • Dyes and their properties
    • Theory of Staining
    • Staining Techniques with haemotoxlin and cosin
    • Mounting of Sections
    • Common Special Stains


    • Fixation
    • Decalification
    • Detection of end point
    • Neutralization and processing


    • Types of specimen and preservation
    • Preparation and fixation of
    • Papanicolaou Staining Techniques
    • Sex Chromatin Staining
    • Museum
      • Reception of specimen
      • Preparation of fixation
      • Restoration of colour
      • Presevation
      • Presentation


    • Autopsy Technique
    • Assisting in Autopssy
    • Preservation of organs & Processing of
    • Waste disposal and safety in


    Ø Practical

    ü Pathology Practicals :


    • Clinical Pathology :
    • Use of Microscope & Care
    • Haemoglobin estimation
    • ESR
    • RBC Count
    • WBC Count
    • Platelet Count
    • Absolute Eosinophil Count
    • Reticulocyte Count
    • PCV
    • Leishman Staining and PBF – Normal and abnormal Cells
    • Bleeding time
    • Clotting time
    • Bone Marrow Aspiration – Staining, Staining for Iron Stores
    • Prothrombin Time – PTI
    • Tests for G6PD deficiency
    • Fowtal Haemoglobin Estimation
    • Serum / Urine Electrophoresis
    • Coombs


      Urine Examinations

    • Physical Examination Colour Reaction Odour Specific gravity Urinary Volume


      Chemical Examination

    • Tests for protein, 24 hours Urinary proteins
    • Bence Jones Proteins
    • Tests for sugar, Ketone bodies
    • Urine for bile salts, bile pigments and Urobilinogen
    • Microscopic examination of urine
    • Semen Analysis.


    ü Hestotechnology Practicals

    • Fixation Processing, Embedding, Section cutting and preparation of
    • Staining of slides H&E Reticulin, PAS Masson Trichrome
    • Sharpening of knives for microtomes
    • Preparation of adhesive to fix the section to the


    Cytology Practicals

    • Collection of samples for cytological examination of various body fluids
    • Preparation and fixation of cytology smears. Giemsa and papanicolaon staining technique
    • Sex Chromatin technique
    • FNAC
    • Blood Bank
    • Theory
      • Introduction and Historical aspects
      • Human Blood Group Antigens, their inheritance and antibodies
      • ABO Blood Group System
      • Sub Groups
      • Source of Antigens, types of


    • Blood Group System.
    • Momenclature and types of Antigens
    • Mode of inheritance
    • Types of antibodies


    • Other Blood Group System


    • Techniques of Grouping and Cross


    • Blood Collection
    • Selection and Screening of
    • Collection of Blood
    • Various anticoagulants used
    • Storage of


    • Blood
    • Procedures and Complications
    • Blood Transfusion Reaction, Types, Investigation and Presentation of Transfusion
    • Coomb’s test.
    • Organisation, operation and Administration and Blood





    • ABO
    • Slide Technique
    • Tube technique


    • Cross
    • Methods of major Cross Matching


    • Typing.
    • Rapid Tube Test
    • Saline Anti D
    • One Stage Albumin Technique
    • Two Stage Albumin technique
    • Coomb’s antihuman Globulin technique


    • Coomb’s
      • Direct Coombs
      • Indirect Coombs


    • Donor Screening and
      • Identification
      • Recording
      • Haemoglobin estimation
      • Relevant Medical History of the Donor
      • Grouping and Typing of Donor’s Blood


    • Drawing of
      • Asepsis
      • Reassurance
      • Vein Puncture re and Collection
      • Care of Donor


    • Blood
      • Anticoagulants preparation
      • Recording the details and storage of Blood
      • Maintenance and cleaning of various equipments used in Blood

    Ø Laboratory Management and Ethics


    • Role of the Laboratory in the Health Care Delivery System :
    • General
    • Human Health &
      • Types of Diseases
      • Process of Diagnosis
      • Laboratory at different levels
      • Duties and responsibilities of Laboratory Personnel


    • Laboratory Service in the Health Care Delivery System in India :
    • Laboratory Service in India
    • The Health Administration System in India
      • At the National Level\
      • At the State Level
      • At the District Level
      • At the Village Level
      • Voluntary Health Organisation in India



    • Laboratory Planning :
    • General Principals
    • Laboratory Goals
    • Operational Data
      • Market Potential
      • Hospital / Laboratory relatives
      • Competitions
      • Laboratory Trends
      • Planning at different levels
      • Guiding Principles for planning Hospital laboratory Services


    • Factors
    • Guiding Principles for Planning
    • Functional Criteria
    • Operational Demnad
    • Sections of a Hospital Laboratory
    • Common Area
    • Design Aspect
    • Space

    Planning for a basic health Laboratory.



    • Laboratory organization (Laboratory Management Techniques) :
    • General Principles
    • Components and Functions of a laboratory
    • Staffing the Laboratory
    • Job descriptions
    • Job specification
    • Work Schedule
    • Personnel re-arrangement and work load
    • Care of Laboratory Glassware, Equipments and Instruments and Chemicals etc :
    • General Principles
    • Care and Cleaning of Glassware
    • Making simple glass wares in Laboratory
    • Care of equipments, Instruments and apparatus etc
    • Laboratory Chemicals their proper use and care


    • Specimen Handeling :
    • General Principles
    • Collection Techniques and containers for specimen
    • Types of specimens
    • Specimens entry
    • Specimens transfer and distribution and re-assignment
    • Specimens disposal
    • Specimens


    • Laboratory Safety :
    • General Principles
    • Laboratory
    • Safety Programmes
    • First Aid

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